Students in the Lewis group receive training on a wide range of modern research equipment. Particularly notable in this regard are the chromatography systems, automated liquid handling, colony picking, and reaction screening systems, and glove boxes maintained in the Lewis labs. Students also receive training in the Physical Biochemistry Instrumentation Facility, the Macromolecular Crystallography Facility, and the Laboratory for Biological Mass Spectrometry at IU. Using and maintaining these systems prepares students for careers across a broad swath of molecular science.
Our labs are equipped with 8’ Fume Hoods, rotovap cabinets, biosafety cabinets, incubators, and all manner of standard equipment for chemical synthesis, molecular cloning, and cell culture. Click here for a virtual lab tour!
We have a range of instruments for preparative and analytical chromatographic separation of small molecules and biomolecules. These include an Agilent 1100 HPLC, an Agilent 1290 UHPLC, an Agilent Agilent 1260 SFC, a Biotage Isolera, and an ÄKTA Fast Protein Liquid Chromatography System.
We also have several instruments dedicated to directed evolution and high throughput screening. These include a Norgren Systems CP-7200 Colony Picker and a Hamilton Nimbus Liquid Handling Robot with an integrated Tecan M200 Pro Plate Reader, all integrated by a Thermo Spinnaker robotic arm. You can see this system in action below.
Finally, we have an IT Double Glove Box with plumbed solvent from an externally accessible IT Solvent Purification System for synthesizing air and water sensitive compounds. We have an IT Single Wet box for working with air senstive proteins and other aqueous species.