Publications
Artificial Metalloenzymes Biocatalysis Chemical Biology Organometallics Other*corresponding author #undergraduate researcher
79
Selective Phosphorylation of Phenols and Benzenediols by the Kinase PsiK and Variants Thereof
Kim, A.; Morato, N. M.; Saha, P. Eyimegwu, P. N.; Niyaz, A. A.; Huang, R.; Cooks, R. G.; Phelan, R. M.; Lewis, J. C. Selective Phosphorylation of Phenols and Benzenediols by the Kinase PsiK and Variants Thereof. Submitted. Available on ChemRxiv: https://doi.org/10.26434/chemrxiv-2025-765rs
Category: Biocatalysis
78
Advancing (-)-Premarineosin A as a Potent Antimalarial Therapeutic via Metabolic Engineering and Late-Stage Derivatization
2) McBride, C. M.; McCauley, M.; Harris, N. R.; Amin, S.; Verhey-Henke, L.; Teklemichael, A. A.; Oliphant, E. N.; Dranchak, P.; Curtis, B. J.; Lev, K. L.; Qu, F.; Snodgrass, H. M.; Lewis, J. C.; Inglese, J.; Su, X.; Pereira, F.; Sherman, D. H. Advancing (-)-Premarineosin A as a Potent Antimalarial Therapeutic via Metabolic Engineering and Late-Stage Derivatization. Submitted. bioRxiv 2025.01.28.635342.
Category: Biocatalysis
77
Identifying and Engineering Flavin Dependent Halogenases for Selective Biocatalysis
Lewis, J. C.* Identifying and Engineering Flavin-Dependent Halogenases for Selective Biocatalysis, Acc. Chem. Res. 2024, accepted
You can download the final peer-reviewed manuscript at ChemRxiv.
Category: Biocatalysis
76
Selective C-H Halogenation of Alkenes and Alkynes Using Flavin-Dependent Halogenases
Jiang, Y.; Kim, A.; Olive, C.; Lewis, J. C.* Selective C-H Halogenation of Alkenes and Alkynes Using Flavin-Dependent Halogenases. Angew. Chem. Int. Ed., accepted
Original preprint available on ChemRxiv.Category: Biocatalysis
75
Non-Native Intramolecular Radical Cyclization Catalyzed by a B12-Dependent Enzyme
Li, J.; Kumar, A.; Lewis, J. C.* Non-Native Intramolecular Radical Cyclization Catalyzed by a B 12-Dependent Enzyme. Angew. Chem. Int. Ed., 2023, e202312893.
Original preprint available on ChemRxiv.
Category: Biocatalysis
74
Non-Native Site-Selective Enzyme Catalysis
Mondal, D.; Snodgrass, H. M.; Gomez, C. A.; Lewis, J. C.* Chem. Rev. 2023, 123, 10381-10431.
See the original preprint available on ChemRxiv.
Category: Artificial Metalloenzymes | Biocatalysis
72
Asymmetric Catalysis by Flavin Dependent Halogenases
Jiang, Y.; Lewis, J. C.* Chirality, 2023, 1-9.
See the original manuscript on ChemRxiv.
Category: Biocatalysis
71
First and Second Sphere Interactions Accelerate Non-Native N-Alkylation Catalysis by the Thermostable, Methanol-Tolerant B12-Dependent Enzyme MtaC.
Kumar, A.; Yang, X., Li, J.; Lewis, J. C.* Chem. Commun., 2023, 59, 4798-4801.
See the original preprint on ChemRxiv.
Category: Biocatalysis | Organometallics
70
Directed Evolution of a Fe(II)- and alpha-Ketoglutarate-Dependent Dioxygenase for Site-Selective Azidation of Unactivated Aliphatic C-H Bonds
Gomez, C.; Mondal, D.; Du, Q.; Chan, N.; Lewis, J. C.* Angew. Chem. Int. Ed. 2023, e202301370.
See the original manuscript on ChemRxiv.
Category: Biocatalysis
69
The Single Component Flavin Reductase/Flavin Dependent Halogenase AetF is a Versatile Catalyst for Selective Bromination and Iodination of Arenes and Olefins
Jiang, Y.; Snodgrass, H. M.; Zubi, Y. S.; Roof, C. V.; Guan, Y.; Mondal, D.;Honeycutt, N. H.#; Lee, J.; Lewis, R. D.; Martinez, C. A.; Lewis, J. C.* Angew. Chem. Int. Ed. 2022, 61, e202214610.
See the original manuscript on ChemRxiv.
Category: Biocatalysis
68
Expanding the Reactivity of Flavin Dependent Halogenases Toward Olefins via Enantioselective Intramolecular Haloetherification and Chemoenzymatic Oxidative Rearrangements
Jiang, Y.; Mondal, D.; Lewis, J. C.* ACS Catalysis, 2022, 12, 13501-13505. Check out the original manuscript on ChemRxiv.
Category: Biocatalysis
67
Non-native Anionic Ligand Binding and Reactivity in Engineered Variants of the Fe(II)- and α-Ketoglutarate-Dependent Oxygenase, SadA
Chan, N. H.; Gomez, C.; Vennelakanti, V.; Du, Q.; Kulik, H. J.*; Lewis, J. C.* Inorg. Chem. 2022, 61, 14477-14485. Check out the original manuscript on ChemRxiv.
Category: Biocatalysis
65
Directed Evolution of Flavin-Dependent Halogenases for Atroposelective Halogenation of 3-Aryl-4(3H)-quinazolinones via Kinetic or Dynamic Kinetic Resolution
Snodgrass, H. M.; Mondal, D.; Lewis, J. C.* J. Am. Chem. Soc. 2022, 144, 16676-16682. See the original manuscript on ChemRxiv.
Category: Biocatalysis
64
Analysis of Laboratory-Evolved Flavin-Dependent Halogenases Affords a Computational Model for Predicting Halogenase Site Selectivity
Andorfer, M. C.; Evans, D.; Yang, S. He, C. Q.;Girlich, A. M.#; Vergara-Coll, J.#; Sukumar, N.; Houk, K. N.*; Lewis, J. C.* Chem. Catal. 2022, 2, 2658-2674. Check out the original manuscript on ChemRxiv.
Category: Biocatalysis
63
Metal-Responsive Regulation of Enzyme Catalysis Using Genetically Encoded Chemical Switches
Zubi, Y. S.; Seki, K.; Li, Y.; Hunt, A.; Liu, B.; Roux, B.*, Jewett, M. C.*, Lewis, J. C.* Nat. Commun. 2022, 13, 1864. Preprint available on ChemRxiv. See peer review here.
Category: Artificial Metalloenzymes | Biocatalysis | Chemical Biology
61
Controlling Non-Native B12 Reactivity and Catalysis in the Transcription Factor CarH
Yang, X.; Gerroll, B. H. R.; Jiang, Y.; Kumar, A.; Zubi, Y. S.; Baker, L. A.; Lewis, J. C.* ACS Catal. 2022, 12, 935-942. Preprint available on ChemRxiv.
Category: Biocatalysis | Organometallics
60
Phage-Assisted Continuous Evolution and Selection of Enzymes for Chemical Synthesis
Jones, K. A.; Snodgrass, H. M.; Belsare, K.; Dickinson, B. C.*, Lewis, J. C.* ACS Central Science, 2021, 7, 1581-1590. Preprint available on ChemRxiv.
Category: Biocatalysis | Chemical Biology
58
Flavin-Dependent Halogenases Catalyze Enantioselective Olefin Halocyclization.
Dibyendu Mondal, Brian F. Fisher, Yuhua Jiang, Jared C. Lewis*. Flavin-Dependent Halogenases Catalyze Enantioselective Olefin Halocyclization. Nat. Commun. 2021, 3268. You can read the original manuscript at ChemRxiv. See peer review discussion here.
Category: Biocatalysis
55
A High-throughput Method for Directed Evolution of NAD(P)+ dependent Dehydrogenases for the Reduction of Biomimetic Nicotinamide Analogues
Huang, R.; Chen, H.; Upp, D. M.; Lewis, J. C.; Zhang, Y.-H. P. J.* A High-throughput Method for Directed Evolution of NAD(P)+-dependent Dehydrogenases for the Reduction of Biomimetic Nicotinamide Analogues. ACS Catalysis, 2019, 9, 11709.
Category: Biocatalysis
53
Site-Selective C-H Halogenation using Flavin-Dependent Halogenases Identified via Family-Wide Activity Profiling
Fisher, B. F.; Snodgrass, H. M.; Jones, K. A.; Andorfer, M. C.; Lewis, J. C.* Site-Selective C-H Halogenation using Flavin-Dependent Halogenases Identified via Family-Wide Activity Profiling. ACS Central Science, 2019, 11, 1184. See the original manuscript at ChemRxiv.
Category: Biocatalysis
49
Pyrococcus furiosus Prolyl Oligopeptidase: A Dynamic Supramolecular Host for Peptidase and Dirhodium Catalysis
Ellis-Guardiola, K.; Rui, H.; Beckner, R.;# Srivastava, P.; Sukumar, N.*; Roux, B.*; Lewis, J. C.* Crystal Structure and Conformational Dynamics of Pyrococcus furiosus Prolyl Oligopeptidase. Biochemistry, 2019, 58, 1616.
The studies detailed in this manuscript were originally reported on ChemRxiv. The ChemRxiv manuscript detailed how the large scale conformational dynamics outlined in the Biochemistry paper can be used to rationalize the specificity of dirhodium ArMs created from the POP scaffold for cyclopropanation over water O-H insertion. Unfortunately, we were unable to convince reviewers of the latter point, but you can still read about it on ChemRxiv here!
Category: Artificial Metalloenzymes | Biocatalysis
48
Enantioselective Desymmetrization of Methylenedianilines via Enzyme-Catalyzed Remote Halogenation
Payne, J. T.; Butkovich, P.; Kunze, K. N. #; Park, H.-J.; Yang, D.-S.; Lewis, J. C.* Enantioselective Desymmetrization of Methylenedianilines via Enzyme-Catalyzed Remote Halogenation. J. Am. Chem. Soc. 2018, 140, 546-549.
Category: Biocatalysis
45
Understanding and Improving the Activity of Flavin Dependent Halogenases via Random and Targeted Mutagenesis
Andorfer, M. C.; Lewis, J. C.* Understanding and Improving the Activity of Flavin Dependent Halogenases via Random and Targeted Mutagenesis. Ann. Rev. Biochem. 2018, 87, 159-185.
Category: Biocatalysis
42
Aromatic Halogenation Using Bifunctional Flavin Reductase-Halogenase Fusion Enzymes
Andorfer, M. C.; Belsare, K. D.; Girlich, A. M. #; Lewis, J. C.* Aromatic Halogenation Using Bifunctional Flavin Reductase-Halogenase Fusion Enzymes. ChemBioChem, 2017, 18, 2099-2103.
Category: Biocatalysis
40
Understanding Flavin-Dependent Halogenase Reactivity via Substrate Activity Profiling
Andorfer, M. C.; Grob, J. E.; Hajdin, C. E.; Chael, J. R. #; Siuti, P.; Lilly, J.; Tan, K. L.*; Lewis, J. C.* Understanding Flavin-Dependent Halogenase Reactivity via Substrate Activity Profiling. ACS Catalysis. 2017, 7, 1897-1904.
Category: Biocatalysis
39
A Simple Combinatorial Codon Mutagenesis Method for Targeted Protein Engineering
Belsare, K.; Andorfer, M. C.; Cardenas, F. #; Chael, J. R. #; Park, H. J.; Lewis, J. C.* A Simple Combinatorial Codon Mutagenesis Method for Targeted Protein Engineering. ACS Synth. Biol. 2017, 6, 416-420.
Category: Biocatalysis
37
Engineering Flavin-Dependent Halogenases
Payne, J. T.; Andorfer, M. C.; Lewis, J. C.* Engineering Flavin-Dependent Halogenases. Meth. Enz. 2016, 575, 93-126.
Category: Biocatalysis
36
Directed Evolution of RebH for Catalyst-Controlled Halogenation of Indole C-H Bonds
Andorfer, M. C.; Park, H. J.; Vergara-Coll, J. #; Lewis, J. C.* Directed Evolution of RebH for Catalyst-Controlled Halogenation of Indole C-H Bonds. Chem. Sci. 2016, 7, 3720-3729.
Category: Biocatalysis
35
Late-Stage Diversification of Biologically Active Molecules via Chemoenzymatic C-H Functionalization
Durak, L. J.; Payne, J. T.; Lewis, J. C.* Late-Stage Diversification of Biologically Active Molecules via Chemoenzymatic C-H Functionalization. ACS Catal. 2016, 6, 1451-1454.
Category: Biocatalysis
32
Directed Evolution of RebH for Site Selective Halogenation of Large, Biologically Active Molecules
Payne, J. T.; Poor, C. B.; Lewis, J. C.* Directed Evolution of RebH for Site Selective Halogenation of Large, Biologically Active Molecules. Angew. Chem. Int. Ed. 2015, 54, 4226.
See a Synfacts comment here.
Category: Biocatalysis
30
Improving the Stability of the FAD-Dependent Halogenase RebH Using Directed Evolution
Poor, C. B.; Andorfer, M. C.; Lewis, J. C.* Improving the Stability of the FAD-Dependent Halogenase RebH Using Directed Evolution. ChemBioChem. 2014, 15, 1286-1289.
Category: Biocatalysis
28
Upgrading Nature’s Tools: Expression Enhancement and Preparative Utility of the Halogenase RebH
Payne, J. T.; Lewis, J. C.* Upgrading Nature’s Tools: Expression Enhancement and Preparative Utility of the Halogenase RebH. Synlett 2014, 25, 1345-1349.
Category: Biocatalysis
23
Regioselective Arene Halogenation Using the FAD-Dependent Halogenase RebH
Payne, J. T.; Andorfer, M. C.; Lewis, J. C. Regioselective Arene Halogenation Using the FAD-Dependent Halogenase RebH. Angew. Chemie. Int. Ed. 2013, 125, 5379-5382.
Category: Biocatalysis
21
Enantioselective Intramolecular C-H amination Catalysed by Engineered Cytochrome P450 Enzymes in vitro and in vivo
McIntosh, J. A.; Coelho, P. S.; Farwell, C. C.; Wang, Z. J.; Lewis, J. C.; Brown, T. R. #; Arnold, F. H.* Enantioselective Intramolecular C-H amination Catalysed by Engineered Cytochrome P450 Enzymes in vitro and in vivo. Angew. Chem. Int. Ed. 2013, 52, 9309 –9312.
Category: Biocatalysis
20
Synthetic Biology Approaches for Organic Synthesis
“Synthetic Biology Approaches for Organic Synthesis,” P. S. Coelho, J. C. Lewis, F. H. Arnold.* Article 00931 in Comprehensive Organic Synthesis II. G. Molander and P. Knochel (Eds.), Elsevier Ltd: Oxford. 2014, 390-420.
Category: Biocatalysis
19
Enzymatic Functionalization of Carbon-Hydrogen Bonds
Lewis, J. C.; Coelho, P. S.; Arnold, F. H.* Enzymatic Functionalization of Carbon-Hydrogen Bonds. Chem. Soc. Rev. 2011, 40, 2003-2021.
Category: Biocatalysis
18
Combinatorial Alanine Substitution Enables Rapid Optimization of Cytochrome P450BM3 for Selective Hydroxylation of Large Substrates
Lewis, J. C.; Mantovani, S. M.; Fu, Y.; Snow, C. D.; Komor, R. S.; Wong, C. H.; Arnold, F. H.* Combinatorial Alanine Substitution Enables Rapid Optimization of Cytochrome P450BM3 for Selective Hydroxylation of Large Substrates. ChemBioChem. 2010, 11, 2502-2505.
Category: Biocatalysis
17
Chemoenzymatic Elaboration of Monosaccharides Using Engineered Cytochrome P450BM3 Demethylases
Lewis, J. C.; Bastian, S.; Bennett, C. S.; Fu, Y.; Mitsuda, Y.; Chen, M. M.; Greenberg, W. A.; Wong, C.-H.; Arnold, F. H.* Chemoenzymatic Elaboration of Monosaccharides Using Engineered Cytochrome P450BM3 Demethylases. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 16550-16555.
Category: Biocatalysis
16
Catalysts on Demand: Selective Oxidations by Laboratory-Evolved Cytochrome P450 BM-3
Lewis, J. C.; Arnold, F. H.* Catalysts on Demand: Selective Oxidations by Laboratory-Evolved Cytochrome P450 BM-3. Chimia 2009, 63, 309-312.
Category: Biocatalysis
Patents
3 Coelho, Pedro, S.; Brustad, Eric M.; Arnold, Frances H.; Wang, Z.; Lewis, Jared C. In vivo and in vitro olefin cyclopropanation catalyzed by engineered and chimeric heme enzyme.” PCT Int. Appl. WO2014058744, 2014.