Biocatalysis – The Lewis Group

Expanding the Reactivity of Flavin Dependent Halogenases Toward Olefins via Enantioselective Intramolecular Haloetherification and Chemoenzymatic Oxidative Rearrangements

Jiang, Y.; Mondal, D.; Lewis, J. C.* Submitted. Preprint available on ChemRxiv.

Non-native Anionic Ligand Binding and Reactivity in Engineered Variants of the Fe(II)- and α-Ketoglutarate-Dependent Oxygenase, SadA

Chan, N. H.; Gomez, C.; Vennelakanti, V.; Du, Q.; Kulik, H. J.*; Lewis, J. C.* Submitted. Preprint available on ChemRxiv.

Directed Evolution of Flavin-Dependent Halogenases for Atroposelective Halogenation of 3-Aryl-4(3H)-quinazolinones via Kinetic or Dynamic Kinetic Resolution

Snodgrass, H. M.; Mondal, D.; Lewis, J. C.* Submitted. Preprint available on ChemRxiv.

Analysis of Laboratory-Evolved Flavin-Dependent Halogenases Affords a Computational Model for Predicting Halogenase Site Selectivity

Andorfer, M. C.; Evans, D.; Yang, S. He, C. Q.; Girlich, A. M.#; Vergara-Coll, J.#; Sukumar, N.; Houk, K. N.*; Lewis, J. C.* Chem. Catal. 2022. Check out the original manuscript on ChemRxiv.

Metal-Responsive Regulation of Enzyme Catalysis Using Genetically Encoded Chemical Switches

Zubi, Y. S.; Seki, K.; Li, Y.; Hunt, A.; Liu, B.; Roux, B.*, Jewett, M. C.*, Lewis, J. C.* Nat. Commun. 2022, 13, 1864. Preprint available on ChemRxiv. See peer review here.

Controlling Non-Native B12 Reactivity and Catalysis in the Transcription Factor CarH

Yang, X.; Gerroll, B. H. R.; Jiang, Y.; Kumar, A.; Zubi, Y. S.; Baker, L. A.; Lewis, J. C.* ACS Catal. 2022, 12, 935-942. Preprint available on ChemRxiv.

Phage-Assisted Continuous Evolution and Selection of Enzymes for Chemical Synthesis

Jones, K. A.; Snodgrass, H. M.; Belsare, K.; Dickinson, B. C.*, Lewis, J. C.* ACS Central Science, 2021, 7, 1581-1590. Preprint available on ChemRxiv.

Flavin-Dependent Halogenases Catalyze Enantioselective Olefin Halocyclization.

Dibyendu Mondal, Brian F. Fisher, Yuhua Jiang, Jared C. Lewis*. Flavin-Dependent Halogenases Catalyze Enantioselective Olefin Halocyclization. Nat. Commun. 2021, 3268. You can read the original manuscript at ChemRxiv. See peer review discussion here.

A High-throughput Method for Directed Evolution of NAD(P)+ dependent Dehydrogenases for the Reduction of Biomimetic Nicotinamide Analogues

Huang, R.; Chen, H.; Upp, D. M.; Lewis, J. C.; Zhang, Y.-H. P. J.* A High-throughput Method for Directed Evolution of NAD(P)+-dependent Dehydrogenases for the Reduction of Biomimetic Nicotinamide Analogues. ACS Catalysis, 2019, 9, 11709.

Site-Selective C-H Halogenation using Flavin-Dependent Halogenases Identified via Family-Wide Activity Profiling

Fisher, B. F.; Snodgrass, H. M.; Jones, K. A.; Andorfer, M. C.; Lewis, J. C.* Site-Selective C-H Halogenation using Flavin-Dependent Halogenases Identified via Family-Wide Activity Profiling. ACS Central Science, 2019, 11, 1184. See the original manuscript at ChemRxiv.

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